Animal Resource Center:  The University of Chicago Animal Resources Center (ARC) has a PHS assurance with OLAW and is a USDA registered research facility. The animal care program has been accredited by AAALAC International since 2002. There are 6 full time veterinarians, including the Attending Veterinarian, Dr. George Langan, who support the animal care and use program at The University of Chicago. All but one of the veterinarians are board certified by the American College of Laboratory Animal Medicine. There are 3 veterinarians participating in our lab animal medicine residency. In addition, there are 7 veterinarian technicians who along with the veterinarians are responsible for the veterinary care for all animals at the University.

Biostatistics: This facility is closely associated with the Department of Health Studies. The Biostatistics Laboratory provides University researchers with biostatistical, epidemiological, and health services research expertise in a collaborative setting.

Cellular & Tissue-based Processing cGMP:The mission of the Cellular and Tissue-Based Processing cGMP facility, located in Kovler Laboratory is to serve as a resource and to support investigators in meeting requirements set by the FDA for cell, gene and tissue translational therapies for Phase I and II human clinical trials. The cGMP facility provides a clean-room environment in which to transition research from the laboratory bench to clinical phase trials.

DNA Sequencing and Genotyping: The facility operates two Applied Biosystems 3730XL and one 3130XL genetic analyzers for small and high throughput users. Moderate- to high-throughput genotyping is handled by a Sequenom MassArray system. The core offers shot-gun subcloning of large genomic templates with optional downstream high-throughput sequencing, and offers a miniprepping service for moderate to high-copy plasmids. Technical consultations for DNA preparation and interpretation of results, as well as archival data storage, are provided at no charge.

Electron Microscopy: The Electron Microscopy is a joint BSD/PSD facility. Users have access to an FEI Tecnai F30 scanning/transmission electron microscope. The facility is located in ESB23 in the Center for Integrative Science. The group provides preparation and viewing services for transmission electron microscopy.

Flow Cytometry: The facility is designed to meet the wide-spread needs for specialized cytologic analysis and continues to respond to the demand for new and improved technology. The primary location, located in 037 Kovler Laboratory houses 2 high-speed cell sorters (DakoCytomation MoFlo HTS and BD FACSAria) and 3 bench top analyzers (Two BD LSR II instruments and a BD FACScan). The facility maintains 3 satellite locations with bench top instrumentation: S319 AMB: BD FACSCanto and BD FACSCalibur HTS with high throughput capability; R111 BSLC: BD FACSCanto; R409 BLSC: FACSCanto. The main facility also has ancillary fluorescence detection equipment including a BioPlex system for bead based analytic assays, an ELISpot analyzer and a fluorescence plate reader. Fluorescence microscopes complement the analytical capacity of the flow cytometers.

Functional Genomics: The Functional Genomics Facility, located in G405 AMB provides resources for investigating gene function with a focus on applying microarray and oligonucleotide technology. As a service facility, the FGF provides expertise and equipment for all phases of array-based services, ranging from sample preparation through to data analysis and management for UC investigators as well as qualified external users. Facility equipment includes a variety of Affymetrix microarray platforms, ABI Real-Time PCR technologies, Codelink arrays, Agilent DNA Microarray platform, and a variety of customized services and data analysis support.

Immunohistochemistry: The IHC Facility is located in 051 Kovler. It provides the following services for human and animal research tissues: tissue embedding, frozen and paraffin tissue sectioning, tissue staining (H&E and up to 3-color immuno-staining), in situ hybridization, development of novel antibody panels and references and expertise regarding IHC and tissue procurement.

Integrated Microscopy: The facility can accommodate live cell imaging in multiple formats. The N710 location houses 4 bright-field and fluorescence light microscopes (Axioplan, Axioskop, Axiovert 100tv, and Axiovert 200m) and a stereomicroscope. The Abbott 120/129 location houses multiple microscopes including a Leica SP2 Laser scanning microscope, an Olympus Fluoview laser scanning microscope, the Olympus IX81 live-cell microscope, a Leitz upright scope, an Axiovert 135, Leica SP5 2 photon confocal microscope and an Olympus DSU spinning disk confocal.

Small Animal Imaging: The Functional & Molecular Imaging Facility is developing multiple imaging modalities for rodents and small primates. This group of facilities include the existing MRIS and Optical Imaging labs and the developing modalities of Ultrasound, micro-PET, CT and SPECT.

Transgenic Mice/Embryonic Stem Cell Facility: The core facility provides a large number of services to University of Chicago investigators including: transgenic mouse production, ES cell technology mouse production (from ES culture, gene targeting through blastocyst injection), timed mouse pregnancies, and embryo rederivation in conjunction with the Animal Resources Center (ARC). We also provide timed pregnant females of various strains and other mouse breeding services. The facility maintains microinjection microscopes as well as surgical dissection scopes. The facility is located in the hospital complex with the animal procedure facilities located within the Animal Resource Center barrier facility.